A simple, accurate and precise dual wavelength UV spectrophotometric method was developed for simultaneous determination of paracetamol and tramadol in combined pharmaceutical dosage forms. The literature review reveals that there was no dual wavelength method developed for this combination of drugs, hence this method was developed. The wavelengths selected for determination of paracetamol were 255 nm and 282 nm, whereas, the wavelengths selected for determination of tramadol were 230.59 nm and 252.94 nm. Methanol and distilled water were used as the solvents. Regression analysis of Beer’s plots showed good correlation in concentration range of 2-35 μg/ml for paracetamol and 2-30 μg/ml for tramadol. Accuracy of method was found between 98.0-102.0%. The precision (intra-day, inter-day and analyst to analyst) of method was found within limits (% CV<2). LOD was found to be 0.10 μg and 0.63 μg for paracetamol and tramadol respectively and LOQ was found to be 0.30 μg and 1.92 μg for paracetamol and tramadol respectively. The proposed method was successfully applied to determination of these drugs in laboratory-prepared mixtures and commercial tablets.
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